Pretend you need to analyze solutions that contain variable kinds and amounts of amino acids ( not whole proteins or peptides ). To do this analysis , you need a practical method that can separate and quantitatively detect all twenty fundamental – amino acids . Briefly discuss one procedure , other than paper chromatography \/ ninhydrin , that could be used to solve this analytical problem . It’s okay if your procedure requires more than one step ( ie a separation technique followed by an analytical step ).<\/p>\n
Include in your answer : <\/p>\n
a ) a description of the molecular basis for the separation chemistry<\/p>\n
b ) a description of the technique that will be used for detection of the analytes and how \/why detection would be quantitative<\/p>\n
c ) the strategy that will be used to characterize which of the twenty amino acids were in a given mixture <\/p>\n
d ) the limitations of the procedure especially regarding whether it would really work for all 20 amino acids and whether it would truly be quantitative . ( for example , if you think it might not separate every amino acid, explain specifically which ones might be left unseparated and why). <\/p>\n
e ) the peer- reviewed reference for your chosen technique (bear in mind that an “open source” reference such as Wikipedia does not count as a peer-reviewed reference)<\/p>\n","protected":false},"excerpt":{"rendered":"
Pretend you need to analyze solutions that contain variable kinds and amounts of amino acids ( not whole proteins or peptides ). To do this analysis , you need a practical method that can separate and quantitatively detect all twenty fundamental – amino acids . Briefly discuss one procedure , other than paper chromatography \/ […]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"open","ping_status":"closed","template":"","meta":[],"disciplines":[275],"paper_types":[],"tagged":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.goodacademic.com\/blog\/wp-json\/wp\/v2\/questions\/7385"}],"collection":[{"href":"https:\/\/www.goodacademic.com\/blog\/wp-json\/wp\/v2\/questions"}],"about":[{"href":"https:\/\/www.goodacademic.com\/blog\/wp-json\/wp\/v2\/types\/questions"}],"author":[{"embeddable":true,"href":"https:\/\/www.goodacademic.com\/blog\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.goodacademic.com\/blog\/wp-json\/wp\/v2\/comments?post=7385"}],"version-history":[{"count":0,"href":"https:\/\/www.goodacademic.com\/blog\/wp-json\/wp\/v2\/questions\/7385\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.goodacademic.com\/blog\/wp-json\/wp\/v2\/media?parent=7385"}],"wp:term":[{"taxonomy":"disciplines","embeddable":true,"href":"https:\/\/www.goodacademic.com\/blog\/wp-json\/wp\/v2\/disciplines?post=7385"},{"taxonomy":"paper_types","embeddable":true,"href":"https:\/\/www.goodacademic.com\/blog\/wp-json\/wp\/v2\/paper_types?post=7385"},{"taxonomy":"tagged","embeddable":true,"href":"https:\/\/www.goodacademic.com\/blog\/wp-json\/wp\/v2\/tagged?post=7385"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}