{"id":10159,"date":"2023-03-23T14:17:08","date_gmt":"2023-03-23T14:17:08","guid":{"rendered":"https:\/\/www.goodacademic.com\/blog\/questions\/discussion-the-lab-report-with-topic-making-hsppa1-alterations-and-introducing-a-dna-vector-sample-of-hsppa1-for-error-prone-and-site-directed-mutations-into-the-bacterial-strain-e-coli\/"},"modified":"2023-03-23T14:17:08","modified_gmt":"2023-03-23T14:17:08","slug":"discussion-the-lab-report-with-topic-making-hsppa1-alterations-and-introducing-a-dna-vector-sample-of-hsppa1-for-error-prone-and-site-directed-mutations-into-the-bacterial-strain-e-coli","status":"publish","type":"questions","link":"https:\/\/www.goodacademic.com\/blog\/questions\/discussion-the-lab-report-with-topic-making-hsppa1-alterations-and-introducing-a-dna-vector-sample-of-hsppa1-for-error-prone-and-site-directed-mutations-into-the-bacterial-strain-e-coli\/","title":{"rendered":"Discussion the lab report with topic: “Making hsPPA1 alterations and introducing a DNA vector sample of hsPPA1 for error-prone and site-directed mutations into the bacterial strain E. coli”"},"content":{"rendered":"

I need help to do the discussion part of my lab report” Making hsPPA1 alterations and introducing a DNA vector sample of hsPPA1 for error-prone and site-directed mutations into the bacterial strain E. coli”. <\/p>\n

there were couple questions that you can use to discuss about the data section:<\/div>\n
-: What are the overall reactions of EcoRV and DpnI, and why were they useful for the cloning strategies in this lab?<\/span><\/div>\n
– Explain the features of DNA spectra and what parts of the DNA molecule are responsible for these features. Include a chemdraw figure demonstrating the Watson-Crick base pairs.<\/span><\/div>\n
– Explain duplication and error rate numbers.<\/div>\n
-Would the experiment have failed if the PNK (Polynucleotide kinase) reaction was omitted? Explain why or why not? <\/div>\n
– Compare the transformation efficiencies result to the value of 0.6 \u2013 1.0 x 109 cfu\/\u00b5g pUC19 reported by New England Biolabs when transforming a 100% circularized pUC19 vector. <\/span>Explain your results.<\/span><\/div>\n
<\/div>\n
I also upload the lab manual of my lab and my lab report on files section: Please look at that before you do my assigment because it was alot of information. Thank you<\/div>\n
<\/div>\n","protected":false},"excerpt":{"rendered":"

I need help to do the discussion part of my lab report” Making hsPPA1 alterations and introducing a DNA vector sample of hsPPA1 for error-prone and site-directed mutations into the bacterial strain E. coli”.  there were couple questions that you can use to discuss about the data section: -: What are the overall reactions of […]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"open","ping_status":"closed","template":"","meta":[],"disciplines":[275],"paper_types":[],"tagged":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.goodacademic.com\/blog\/wp-json\/wp\/v2\/questions\/10159"}],"collection":[{"href":"https:\/\/www.goodacademic.com\/blog\/wp-json\/wp\/v2\/questions"}],"about":[{"href":"https:\/\/www.goodacademic.com\/blog\/wp-json\/wp\/v2\/types\/questions"}],"author":[{"embeddable":true,"href":"https:\/\/www.goodacademic.com\/blog\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.goodacademic.com\/blog\/wp-json\/wp\/v2\/comments?post=10159"}],"version-history":[{"count":0,"href":"https:\/\/www.goodacademic.com\/blog\/wp-json\/wp\/v2\/questions\/10159\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.goodacademic.com\/blog\/wp-json\/wp\/v2\/media?parent=10159"}],"wp:term":[{"taxonomy":"disciplines","embeddable":true,"href":"https:\/\/www.goodacademic.com\/blog\/wp-json\/wp\/v2\/disciplines?post=10159"},{"taxonomy":"paper_types","embeddable":true,"href":"https:\/\/www.goodacademic.com\/blog\/wp-json\/wp\/v2\/paper_types?post=10159"},{"taxonomy":"tagged","embeddable":true,"href":"https:\/\/www.goodacademic.com\/blog\/wp-json\/wp\/v2\/tagged?post=10159"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}